Escherichia coli serogroups in slaughterhouses: Antibiotic susceptibility and molecular typing of isolates

BAREL M., HIZLISOY H., Gungor C., Dishan A., Disli H. B., AL S., ...Daha Fazla

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, cilt.371, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 371
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.ijfoodmicro.2022.109673
  • Dergi Adı: INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, EMBASE, Environment Index, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database, DIALNET
  • Anahtar Kelimeler: Antibiotic resistance genes, E, coli, Serogroups, Virulence genes, POLYMERASE-CHAIN-REACTION, ANTIMICROBIAL RESISTANCE GENES, SHIGA-TOXIN, VIRULENCE FACTORS, FEEDLOT CATTLE, GROUND-BEEF, O157, STRAINS, PREVALENCE, STEC
  • Erciyes Üniversitesi Adresli: Evet

Özet

This study aimed to investigate the contamination of carcasses and slaughterhouse environment with Escherichia coli O157:H7 and non-O157 serogroups (O45:H2, O103:H2, O121:H19, O145:H28, O26:H11, O111:H8). For this purpose, a total of 150 samples (30 carcasses, 30 shredding units, 30 knives, 30 slaughterhouse waste water and 30 wall surfaces) were collected from 5 different slaughterhouses in Kayseri, Turkey. The conventional and molecular methods were performed in order to detect Escherichia coli and its serogroups. Of the 150 samples, 55 (36%) were found to be contaminated with E. coli. Among isolates, E. coli serogroup (O157:H7) were detected in 2 (11%) carcass and 2 (11%) wastewater samples. None of the E. coli isolates harbored tested genes (stx1, stx2, eaeA, and hylA). Effective infection control measures and antibiotic stewardship programs should be adopted to limit the spread of multidrug-resistant bacteria. It was also deduced that these isolates resistance to different antibiotics could be hazardous for public health.