From cattle to pastirma: Contamination source of methicillin susceptible and resistant<i> Staphylococcus</i><i> aureus</i> (MRSA) along the pastirma production chain

Gungor C., BAREL M., Dishan A., Disli H. B., Koskeroglu K., ERTAŞ ONMAZ N.

LWT-FOOD SCIENCE AND TECHNOLOGY, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.lwt.2021.112130
  • Dergi Adı: LWT-FOOD SCIENCE AND TECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Food Science & Technology Abstracts, Veterinary Science Database
  • Anahtar Kelimeler: Cattle, MDR, MRSA, Pastirma production chain, Slaughterhouse, SHOCK-SYNDROME TOXIN-1, ANTIBIOTIC SUSCEPTIBILITY, MULTIPLEX-PCR, ANTIMICROBIAL SUSCEPTIBILITY, MICROBIOLOGICAL QUALITY, EXFOLIATIVE TOXINS, ENTEROTOXINS, GENES, PASTRAMI, PREVALENCE
  • Erciyes Üniversitesi Adresli: Evet

Özet

This study was designed to determine the prevalence of Methicillin Susceptible S. aureus (MSSA) and MethicillinResistant S. aureus (MRSA) and their source of contamination in the pastirma production chain. Additionally, this study was focused on the antimicrobial resistance, virulence profiles, biofilm-forming capabilities and phylogenetic relationships of obtained isolates. A total of 400 samples were analyzed and from which 105 (26.25%) were found positive for coagulase-positive with Staphylococci (CPS). Within the 105 CPS samples, 36 (9%) were identified as S. aureus, from which 8 (2%) were MRSA. Four (11.1%) of 36 S. aureus isolates, of which 3 (37.5%) were MRSA, had a multidrug resistance (MDR), and 6 MRSA strains were found positive for one or more SEs genes (seb, sed, and see). According to the ERIC-PCR analysis, only two S. aureus strains (one with personnel origin and one with carcass origin) were genetically identical. This study highlights the detection frequency of S. aureus in samples analyzed observed, while low, can be a significant public health problem, especially due to the identification of MRSA harboring some enterotoxin genes and having MDR.