Screening of different fruit extracts from Viburnum opulus L. as inhibitors of key enzymes linked to type 2 diabetes and antioxidants: a comparative evaluation


PASAYEVA L., Kararenk A. C., FATULLAYEV H.

JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION, vol.15, no.5, pp.4403-4410, 2021 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 15 Issue: 5
  • Publication Date: 2021
  • Doi Number: 10.1007/s11694-021-01007-4
  • Journal Name: JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, CAB Abstracts, Compendex, Food Science & Technology Abstracts, Veterinary Science Database
  • Page Numbers: pp.4403-4410
  • Keywords: Caprifoliaceae, alpha-Amylase, alpha-Glucosidase, Diabetes, ALPHA-GLUCOSIDASE, ANTIMICROBIAL ACTIVITY, MASS-SPECTROMETRY, PHENOLIC CONTENT, CRANBERRY, GILABURU, CELLS, ACID, AMYLASE, UV
  • Erciyes University Affiliated: Yes

Abstract

In this study, the inhibiting effect on a-amylase and a-glucosidase, the antioxidant activity of different fruit extracts from V. opulus and the chemical compounds in most active extract were investigated. The antioxidant activity was tested with -DPPH., -ABTS.+ and FRAP tests and the phytochemical analyses of the active extract was determined by LC- MS/MS. According to the results, the highest a-glucosidase and a-amylase activity even from positive control acarbose were found in ethanol extract -(IC50 = 3.671 +/- 0.016 mu g/mL and 601.667 +/- 0.015 mu g/mL, respectively). Also, the highest TPC (96.000 +/- 0.001 mgGAE/gextract) and TFC (69.327 +/- 0.693 mgCA /gextract) was found in the same extract and this extract showed the highest antioxidant activity on -DPPH. (%34), FRAP (at a concentration of 1 mg/mL equivalent to 1521.527 +/- 1.528 mmol -Fe2+) and -ABTS+. (at a concentration of 0.5 mg/mL equivalent to 0.43 +/- 0.002 mu M Trolox) tests. Organic and phenolic acids and triterpenic compounds were identified in V. opulus ethyl acetate extract by LC-MS/MS analysis. In conclusion, this study may be supported with detailed investigation on different pathways in anti-diabetic activity and could contribute to the advanced studies of this species.