There are eight centers of origin for cultivated plants and Turkey is located in the interception of two of these centers, the Near East and the Mediterranean. Therefore, Turkey is known to be the gene center for diversification and dispersion of such main cereal crops such as wheat, barley, rye and oat. This study was performed to determine glutenin gene allele frequencies and Glu-1 quality scores of 116 local wheat landraces of Turkish bread wheat. SDSPAGE and PCR were used to identify glutenin gene alleles. The results showed that the studied Turkish local wheat landraces contained a total of 19 different subunits (3 subunits in Glu-Al, 11 in Glu-B1 and 5 in Glu-D1) with 50 different combinations. The highest and the lowest allelic combinations were determined in East Anatolia and the Aegean regions, respectively. Glu-A1c (65.11%), Glu-B lb (53.60%) and Glu-D1a (58.30%) were the most frequent alleles. The Glu-1 quality score was found to be 6.07 for the studied genotypes. Among the regions, the highest (7.18) and the lowest (4.80) mean Glu-1 scores were detected in Marmara and Southeastern Anatolia regions, respectively. 4 accessions (TR32846-6, TR36948-1, TR45105 and TR63536) were reported to have the highest Glu-1 quality score as 10. 6 genotypes (TR45398-4, TR480253, TR33264-6, TR393-5, TR52021-3 and TR45094) had the quality score of 9. Including more new landraces may contribute to discover new Glu-1 alleles.