Çukurova Tarım ve Gıda Bilimleri Dergisi, vol.36, no.1, pp.1-12, 2021 (Peer-Reviewed Journal)
In this study, the optimum microwave assisted extraction (MAE) parameters (solid/liquid ratio,
time and power) determined by Box-Behnken Design (BBD) of Response Surface Methodology
(RSM) for the extraction of antioxidant compounds from grapevine leaves. The BBD was used to
define the effects of independent variables on Total Phenolic Content (TPC), DPPH radical
scavenging activity and Trolox Equivalent Antioxidant Capacity (TEAC). Second-order
polynomial model and regression analysis were used for prediction optimum point. The optimum
conditions for MAE of grapevine leaves were determined as solid/liquid ratio of 30%, power of
300 W and time of 300 sec. All of the models was found valid and significant independent
variables (R2
) were found as 0.9282, 0.9340 and 0.9380 and the predicted experimental value of
design 58.204 mg GAE/ mL extract, 95.905% and 65.123% for TPC, DPPH scavenging activity
and TEAC, respectively. The results of the present showed that MAE of grapevine leaves produce
the extracts with a higher antioxidant values in a shorter time when compared with solvent
extraction.