Akademik Veri Yönetim Sistemi
Tezin Türü: Doktora
Tezin Yürütüldüğü Kurum: Orta Doğu Teknik Üniversitesi, Fen Bilimleri Enstitüsü, Biyokimya Anabilim Dalı, Türkiye
Tezin Onay Tarihi: 2009
Tezin Dili: İngilizce
Öğrenci: HAVVA DİNÇ
Asıl Danışman (Eş Danışmanlı Tezler İçin): İnci Zehra Togan
Eş Danışman: Ebru Saatçi
Özet:
The main aim of this study is to determine genetic diversity of milk protein genes
associated with milk traits, namely beta-casein, kappa-casein and betalactoglobulin,
in native Turkish cattle breeds (Turkish Grey, Eastern Anatolian
Red, Anatolian Black, and Southern Anatolian Red) and Turkish Holstein. Only
11% deviation from the Hardy-Weinberg equilibrium and insignificant Fis values
for the populations were observed, indicating that samples are free of inbreeding.
B alleles of these genes, which are positively related with cheese yield and quality,
seem to be relatively high in native Turkish breeds. Therefore, the results suggest
that milk of Turkish native breeds is advantageous for producing high-quality and
-yield cheese.
A1 allele of beta-casein, which releases a bioactive peptide called BCM-7 after
successive gastrointestinal proteolytic digestions, has been claimed to have adverse
health effects on humans. Another aim of this study is to develop a protocol and
assess the potential detrimental effects of BCM-7 on human peripheral blood cells.
Despite the fact that the results are inconclusive, the optimized experimental
protocol will guide further researchers while judging the effect of BCM-7 on
human health.
Even though A1 beta-casein, which has a low frequency in native Turkish breeds,
and hence BCM-7 have no adverse health effects on humans, this probability
should be enough to keep its frequency low in native cattle breeds. Bulls must be
screened for A1 allele of beta-casein as well as E allele of kappa-casein, which is
absent in native breeds and known to have detrimental effects on cheese quality.