Akademik Veri Yönetim Sistemi
Tezin Türü: Yüksek Lisans
Tezin Yürütüldüğü Kurum: Erciyes Üniversitesi, Fen Bilimleri Enstitüsü, Türkiye
Tezin Onay Tarihi: 2025
Tezin Dili: İngilizce
Öğrenci: NOOR RADHWAN ABDULKADHIM ALBUNSRALLA
Asıl Danışman (Eş Danışmanlı Tezler İçin): Abdurrahman Ayvaz
Eş Danışman: Pınar Sağıroğlu
Özet:
The routine phenotypic methods originally identified the NA22
isolates as Klebsiella pneumoniae. Nevertheless, WGS and NCBI annotation
have subsequently confirmed that the microorganism is an Escherichia coli.
The isolate had been recovered from the bloodstream of a patient and was
resistant to many of the most important classes of antibiotics. A total of
eight contigs >1,000 bp were generated through sequencing on the Oxford
Nanopore platform. Three plasmids, NA22_1, NA22_2, and NA22_3, were identified
using PlasmidFinder. Among them, NA22_3 harbored significant resistance genes,
including blaNDM-4, blaCTX-M-15, and aac(6’)-Ib-cr, along with
the tra operon genes that may facilitate plasmid transfer. Functional
annotation was performed against the PATRIC, CARD, and VFDB databases, and 76
resistance and over 200 virulence, as well as over 100 metabolism and transport
involved subsystems, were found. The antimicrobial susceptibility tests
revealed resistance to β-lactams (including carbapenems), fluoroquinolones,
aminoglycosides, and sulfonamides, which were consistent with the genome
profile. The complete genome sequence is included in BioProject ID:
PRJNA1270365. These results highlight the importance of routine genomic
surveillance of multidrug-resistant strains within hospitals.