Akademik Veri Yönetim Sistemi
3. Uluslararası Veteriner Biyokimya ve Klinik Biyokimya Kongresi, Van, Türkiye, 23 - 25 Mayıs 2024, ss.39-40
ABSTRACT
Aim: This study was performed to investigate the effects of ZnO NPs on malondialdehyde (MDA) levels, super
oxidase dismutase (SOD), glutathione peroxidase (GPx) and caspase-3 (CASP3) activities, tumor necrosis factorα (TNF-α) and interleukin 6 (IL-6), immunoglobulin E, G, M (IgE, IgG, IgM) and zinc (Zn) levels in serum/plasma
and liver tissues of rats.
Material-Metod: Forty Wistar Albino rats; it was divided into five equal groups: control (Group I, received 0.5
ml of physiological saline), 5 (Group II) and 10 mg/kg (Group III), b.w./dayZnO, and 5 (Group IV) and 10 mg/kg
(Group V) b.w/dayZnO NPs were administered i.p. every other day for 14 days.
Results: Plasma MDA levels increased in the 10 mg/kg ZnO, 5 and 10 mg/kg ZnO NP groups. Plasma SOD, GPx,
CASP3, liver GPx activities and serum Zn levels increased in the ZnO and ZnO NP groups. The highest SOD, GPx
and CASP3 activities were detected in the 5 mg/kg ZnO NP group. Although there was an increase in plasma
and liver TNF-α levels of 10 mg/kg ZnO, 5 and 10 mg/kg ZnO NP groups, the highest levels were detected in the
ZnO NP groups. Plasma IgG levels increased, especially in the ZnO NP groups. There was no difference between
the groups in terms of liver SOD, CASP3 activities and Zn levels, and both plasma and liver IL-6, Ig E and M
levels.
Conclusion: In conclusion; 5 and 10 mg/kg ZnO NPs can reduce the negative effects of oxidative stress by
increasing plasma/tissue SOD, GPx and CASP3 activities and TNF-α and IgG levels. The effectiveness of
nanoparticles may vary depending on the synthesis method, concentration and application time; therefore, it
would be useful to conduct studies using different doses of ZnO NPs.
Keywords: Caspase-3, cytokine, immunoglobulin, oxidative stress, zinc oxide nanoparticles.