The antimicrobial activities of the ethyl acetate, acetone, methanol and ethanol extracts of Echinops viscosus DC subsp. bithynicus (Boiss) Rech. and E. microcephalus Sm. were studied by disc diffusion method. These extracts were tested against eight bacteria and four fungi, which revealed various levels of antimicrobial activity. The methanol, ethyl acetate, acetone extracts of EMF showed more antibacterial activity against S. aureus (18-19-18 mm 50 μL-1 inhibition zone) than standart antibiotics (f= 95.765; df= 18; p<0.0001). The methanol extracts of EVF showed antibacterial activity against E. coli equal to standart antibiotics (V30 & E15). The ethyl acetate extracts of EVL showed antibacterial activity against B. megaterium equal to standart antibiotic (V30). The acetone extracts of EVF and EVL showed antimicrobial activity againstM. pusilus close rate to standart antibiotic. E. viscosus and E. microcephalus contain antimicrobial components against different microorganisms, which could be in various pharmaceutical preparations. © 2012 Friends Science Publishers.