Total phenolic contents, antioxidant and antimicrobial activities of methanol extracts of six Senecio species growing in the Black Sea region of Turkey (S. pandurifolius, S. trapezuntinus. S. integrifolius subsp. aucheri, S. hypochionaeus var. argaeus, S. hypochionaeus var. ilkasiensis and S. lorentii) are investigated. The total phenolic content is analysed using Folin-Ciocalteu's reagent of the methanol extracts and varies from 19.54 to 81.78 mg GAE/g dry extract. The methanol extracts of the studied species possess antioxidant properties in the range of 70.07-165.21 mg AAE/g dry extract in phosphomolybdenum assay. S. hypochionaeus var. ilkasiensis extract shows maximum activity with an IC50 of 15.94 mu g/ml in DPPH free radical scavenging assay. All of the Senecio methanol extracts show similar antimicrobial activities against microorganisms tested by agar diffusion methods. Klebsiella pneumoniae is the most sensitive microorganism to the all extracts examined while Escherichia coli and Candida albicans are the most resistant one.