Cotton fibers can be finished in different forms such as fiber, yarn, fabric, and etc. depending on the demanded final product. Absorbent cotton is one of these products. In this study it was aimed to introduce an alternative environmentally friendly method for the finishing of cotton slivers which will be useful in production of absorbent cotton. For this aim, cotton slivers were bioscoured with the help of pectinase enzyme in different concentrations for different durations. Meanwhile the effect of ultrasound has been tested too. Then the bioscoured samples were dyed with two different natural dye sources to obtain naturally colored cotton slivers or ozonated to obtain white cotton slivers. As a summary, it was found that ultrasonic bioscouring can be a way for increasing the hydrophilicity of cotton slivers. Moreover, for naturally dyed and for white cotton slivers, pomegranate peel/green tea based dyeings and ozone application were developed as production methods respectively.