Objective: In this study, investigation of Trichomonas vaginalis prevalence using various methods in patients with complaint of vaginal discharge, and comparison of results with different parameters were aimed. Material and Methods: T. vaginalis was investigated in 535 women who applied to Erciyes University Medical Faculty Department of Obstetrics and Gynecology with the complaint of vaginal discharge and in 482 women who did not complain of discharge. Parasite was investigated with direct microscopic examination, Giemsa staining, acridine orange staining and inoculation to cysteine-peptone-liver-maltose medium in 1017 women whose vaginal smear specimens were obtained. Results: T. vaginalis was detected in 11 (2.06%) women with vaginal discharge and in two (0.41%) women in control group. A statistically significant difference was not found between ages and social statuses of the women and detection of the parasite. When presence of parasite was compared according to the type of the vaginal discharge, a statistically significant correlation was found between having a green-nasty smelling discharge and detection of the parasite. Although the parasite was found negative in two cases (15.5%) with direct microscopic examination, in three cases (23.1%) with Giemsa staining and in six cases (46.2%) with acridine orange staining, it was found positive with culture method. Conclusion: We consider that incidence studies investigating the prevalence of trichomoniosis which is a sexually transmitted disease should be conducted with certain intervals, and the individuals who carry this agent should be detected as well by screening asymptomatic individuals using culture methods along with staining and direct examination.