Akademik Veri Yönetim Sistemi
Turk Hijyen ve Deneysel Biyoloji Dergisi, cilt.79, sa.4, ss.720-729, 2022 (Scopus)
Objective: Endotoxemia is a serious complication that is featured by hypotension, weak tissue perfusion and multiple organ failure that is among the top factors that lead to mortality in hospitalized patients. Apilarnil (drone bee brood) is a honeybee product that has biologically active characteristics. Apilarnil contains 25 - 35% dry matter, 9 - 12% protein, 6 - 10% carbohydrates, 5 - 8 lipids, 3% ash, and other unidentified substances. Additionally, its chemical composition includes vitamins (vitamin A, beta carotene, B1, B6, PP, and choline), minerals (calcium, phosphorus, sodium, zinc, manganese, iron, copper, and potassium), and essential amino acids that cannot be synthesized by humans or other animal organisms. Endotoxemia-induced cardiotoxicity is reported in the recent evidence. In this study, it was aimed to determine whether apilarnil, a bee product, has a protective effect on heart tissue in the case of endotoxic shock, which is one of the major causes of intensive care units, by histopathological and immunohistochemical evaluation of TNF-α and BNP in the heart tissue. Methods: 32 Sprague dawley male rats were randomly divided into four equal groups as control, apilarnil administered group (0.8 g/kg), lipopolysaccharide (LPS) group, and apilarnil together with LPS administered group. Immunohistochemical evaluation was performed to determine the differences in the expression of TNF-α and BNP in the heart tissue. Results: Edema, hemorrhage, and infiltration was observed in the LPS group compared to the control group and groups receiving apilarnil. It was observed that this damage decreased significantly in the group treated with LPS and apilarnil. Tumor necrosis factor-alpha (TNF-α) and brain natriuretic peptide (BNP) expressions were significantly increased in the LPS group, and coadministration of LPS and apilarnil suppressed these increased expression levels. Conclusion: Our data indicate that the protective effects of apilarnil may have a therapeutic effect on heart damage caused by LPS by the anti-inflammatory and antioxidant properties of the components found in apilarnil.