Efficiency of ozone (O3) therapy on experimental acidic skin burns in rats

Creative Commons License

Aslan N. E., Erol H., Balcıoğlu E., Yalçın B.

VETERINARIA MEXICO, vol.11, pp.1-10, 2024 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 11
  • Publication Date: 2024
  • Doi Number: 10.22201/fmvz.24486760e.2024.1203
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, DIALNET
  • Page Numbers: pp.1-10
  • Erciyes University Affiliated: Yes


In this study, it was aimed to investigate the efficacy of O3 therapy in experimentally

induced hydrofluoric acid (HF) skin burns in rats. A total of 20

healthy male Wistar Albino rats (weighing 250-300 g, aged 16 weeks) were

used as the material of the study. They were divided into two groups (as

experimental and control groups) of 10 rats which were housed individually

and fed ad libitum. HF skin burns were induced in all animals. The ozonized

(20 μg O3/mL) liquid vaseline was applied topically for seven days to the

experiment group as well as parallelly, a saline solution was applied to the

control group. In the histopathologic evaluation, inflammation, vascularization,

epithelial regeneration and fibrosis were evaluated. The epithelial proliferation

and collagenization were higher and statistically significant in the

experimental group, while the infiltration was higher and statistically significant

in the control group. As a result of this study, it was observed that

ozone plays an important role in the tissue repair process in chemical burns.

The increase of fibroblast activation and re-epithelization in the experimental

group demonstrated that ozone therapy could be suggested as first aid in HF

skin burns. It was concluded that more studies on ozone are needed to shed

light on the subject.