Seeds of 38 cucurbit, 19 melon and 8 watermelon accessions were taken from the genbank of European University of Lefke, Faculty of Agricultural Sciences and Technologies. There are total 65 accessions collected from different ecological region of Northern Cyprus. Furthermore, evaluation studies of these materials have started. In the present study 38 cucurbit, 19 melon and 8 watermelon accessions, sampled from this germplasm, were tested for resistance to zucchini yellow mosaic virus (ZYMV). Seeding performed into violas with 5 replications and after germination of the genotypes, virus inoculation performed by soft scorching ped method to the cotyledons. Two weeks after inoculation, plants were transferred to the field of Faculty of Agricultural Sciences and Technologies. One week after transplanting, virus symptoms appeared. To categorize plants, 0-9 scale was used and plants were grouped as tolerant, resistant and sensitive. According to the results, No ZYMV was determined on one cucurbit (KAB 03) accession and one melon (KAV 20) accession which may be resistant against to ZYMV.