Enzymes are mostly used for bio-polishing process of fabrics which remove the pills and fuzz from fabric surface in order to improve the smoothness. Fabric properties mainly depend on the yarn properties and the yarn arrangements in the fabric construction. In this study, the effects of bio-polishing process on the performance of viscose knitted fabrics made from different yarn types are investigated. After enzymatic treatment, the relatively higher weight loss is observed from fabrics knitted from ring spun (RS) and siro spun (SS) yarns, than those knitted from oe-rotor spun (OERS) and vortex spun (VS) yarns. The decreasing trend of bursting strength of fabrics after enzymatic treatment shows difference for different yarn types. Fabrics knitted from SS yarns show the highest resistance to abrasion before and after enzymatic treatment.