Micropropagation Protocols of New Prunus Hybrids with Significant Rootstock Potential in Fruit Breeding and Cultivation

Bulunuz Palaz E., Ugur R., YAMAN M.

Erwerbs-Obstbau, cilt.65, sa.5, ss.1359-1364, 2023 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 65 Sayı: 5
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1007/s10341-023-00908-6
  • Dergi Adı: Erwerbs-Obstbau
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, CAB Abstracts, Environment Index, Food Science & Technology Abstracts
  • Sayfa Sayıları: ss.1359-1364
  • Anahtar Kelimeler: Hybrid, In vitro, Micropropagation, Prunus, Rootstock
  • Erciyes Üniversitesi Adresli: Evet

Özet

In vitro micropropagation is the most valid production method used for disease-free, clonal, and mass production of plants developed using unique breeding methods. This study was carried out to establish appropriate protocols for the micropropagation of 10 (Prunus amygdalus) × (P. amygdalus × P. persica) hybrid rootstocks obtained by crossbreeding. The maximum micro shoot and shoot length values were obtained from the medium (MED 11) as 3.92 shoots/explant and 14.59 mm, respectively. The best rooting values were obtained from MS medium supplemented with 1.75 mgL−1 IBA + 0.1 mgL−1 BAP (MED-5) in the rooting study. The highest average root number, root length, and root diameter of shoots 2.79 pcs/explant, 14.36 mm, and 1.15 pcs/explant, respectively, were observed. It is suggested that MS media supplemented with MED-11 (0.1 mg.L−1 IBA + 1.75 mg.L−1 BAP + 0.2 mg.L−1 GA3), MED-12 (0.1 mg.L−1 IBA + 2.0 mg.L−1 BAP + 0.2 mg.L−1 GA3) for regeneration, MED‑5 (1.75 mg.L−1 IBA + 0.1 mg.L−1 BAP), and MED‑6 (2.0 mg.L−1 IBA + 0.1 mg.L−1 BAP) hormone combinations for rooting would be appropriate for the micropropagation of hybrid rootstocks. It is thought that the results of the study can be a guide for new studies to be carried out especially in these species.