Low Concentrations of a Silver-Based Nanocomposite to Manage Bacterial Spot of Tomato in the Greenhouse


Creative Commons License

Strayer A., ÖÇSOY I. , Tan W., Jones J. B. , Paret M. L.

PLANT DISEASE, cilt.100, ss.1460-1465, 2016 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 100 Konu: 7
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1094/pdis-05-15-0580-re
  • Dergi Adı: PLANT DISEASE
  • Sayfa Sayıları: ss.1460-1465

Özet

Bacterial spot, caused by four Xanthomonas spp., is one of the most damaging diseases of tomato worldwide. Due to limited disease management options, growers rely heavily on copper-based bactericides, which are often ineffective due to the presence of copper-resistant Xanthomonas strains. This study was undertaken to characterize the antibacterial activity of a silver-based nanocomposite, Ag-dsDNA-GO, and its potential as an alternative to copper. Ag-dsDNA-GO at rates as low as 10 mu g/ml killed all bacterial cells of copper-tolerant and-sensitive Xanthomonas perforans strains in suspensions containing approximately 10(3) CFU/ml within 15 min of exposure in vitro, whereas equivalent rates of copper (10, 25, and 50 mu g/ml) were unable to significantly reduce populations compared with the untreated control after 24 h of exposure (P = 0.05). All copper concentrations killed the copper-sensitive X. perforans strain but required exposure for >= 1 h. Ag-dsDNA-GO also exhibited antibacterial activity against copper tolerant X. vesicatoria, X euvesicatoria, and X. gardneri strains. In greenhouse studies, tomato plants treated with Ag-dsDNA-GO at either 75 or 100 mu g/ml prior to artificial inoculation significantly reduced disease severity when compared with copper-mancozeb and negative controls (P = 0.05). This study highlights the potential of Ag-dsDNA-GO as an alternative to copper in tomato transplant production.