Schisandrin B Treatment After Disease Induction Slightly Improved Experimental Autoimmune Encephalomyelitis


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Yetkin M. F., Erdem Ş., Azizoğlu Z. B., Demir B. S., Acikgoz E., Çakır M., ...More

European Committee for Treatment and Research in Multiple Sclerosis, Milan, Italy, 11 - 13 October 2023, vol.29, no.3, pp.802-803

  • Publication Type: Conference Paper / Full Text
  • Volume: 29
  • Doi Number: 10.1177/13524585231196195
  • City: Milan
  • Country: Italy
  • Page Numbers: pp.802-803
  • Erciyes University Affiliated: Yes

Abstract


Introduction: Schisandrin B is a bioactive compound naturally found in Schisandra rubriflora. We have previously reported that Schisandrin B, when given prophylactically, improved experimental autoimmune encephalomyelitis (EAE).


 


Objectives/Aims: In the current study, we aimed to study its impact on EAE when given after disease induction, and its in vitro effects on murine lymphocytes and helper T cell differentiation.


 


Methods: CD57BL/6-MOG35-55 and SJL/J-PLP139-151 mice were used for EAE induction. Mouse splenocytes and lymphocytes were used for in vitro experiments.


 


Results: Schisandrin B treatment after 8 days after MOG35-55 immunization marginally affected EAE disease scores and area under curve in C57BL/6 mice. Additionally, Schisandrin B slightly reduced GM-CSF and IL-22 production by CD4+ T cells in the draining lymph nodes in SJL/j mice following PLP139-151 immunization. Although no effect was seen in inguinal lymph nodes, cervical lymph nodes showed reduced IL-2 production by CD4+ or CD8+ T cells, a week after the immunizations in SJL/J mice. Importantly, CD25 expression was slightly higher by Treg cells in the cervical lymph nodes of SJL/J mice a week after the immunizations. On the 9th day of immunizations, infiltrating leukocytes in the central nervous system showed reduced CD3+ and CD8+ T cell frequency, and reduced activation as marked by lower CD25 expression. In vitro experiments showed that Schisandrin B dampened IL-6 and MIP-1α by T cells and splenocytes, respectively, and that it blocked Th1 and Th17 T cell polarization significantly at 10 μM doses.


 


Conclusion: Collectively these results suggest an immunomodulatory role for Schisandrin B and its potential in inflammatory conditions like MS.


 


Disclosure of interest: nothing to disclose