The present study was undertaken (1) to characterize the fatty acid (FA) composition particularly on the concentration of conjugated linoleic acid (2) to investigate of physicochemical properties of Anatolian water buffalo milk, and from six different provinces in Turkey. The fat amount in water buffalo milk samples were in the range of 5.97 +/- 0.30% to 9.19 +/- 0.57% and the mean fat was 6.96 +/- 0.25%. The main individual FA in water buffalo milk were in the order 16:0, 18:1 cis9, 14:0, and 18:0. The linoleic acid (CLA), bovinic acid (cis-9, trans-11), represented 1.09 +/- 0.06 in water buffalo milk. Saturated fatty acids (SFA) were the potent fraction in water buffalo milk fat (70.63 +/- 0.7); MUFA and PUFA were 29.37 +/- 0.7 and 0.2 +/- 0.03, respectively. The data exhibit statistically differences (P <= 0.05) in the proportions of individual FA were detected among different provinces in Turkey. Anatolian water buffalos were poor in terms polyunsaturated FA compared to other water buffalos from different countries. Therefore, these results may provide useful information about the nutrient composition of buffalo milk and further studies are warranted to improve the technological and nutritional characteristics of Anatolian buffalo milk.