GLP-1 and GLP-1-receptor expression profiles in pancreatic islets of Diet Induced Obese (DIO) Mice
Eur. Society of Gene and Cell Therapy (ESGCT) and Spanish Society for Gene and Cell Therapy (SETGyC) Collaborative Congress, Madrid, Spain, 1 - 04 October 2013, (Full Text)
- Publication Type: Conference Paper / Full Text
- Country: Spain
- Erciyes University Affiliated: Yes
Abstract
Glucagon-like peptide-1 (GLP-1) is a gut-derived incretin hormone expressed from intestinal L cells located in distal jejunum, ileum and colon with glucoregulatory actions. Its functions
include but not limited to glucose-enhanced insulin secretion, suppression of glucagon secretion, delay in gastric emptying, and reduction in appetite and food intake causing weight loss. Enhancement of insulin gene expression and its biosynthesis, stimulation of beta cell proliferation and differentiation, and inhibition of beta cell apoptosis are the other known beneficial effects of GLP-1. Despite all these beneficial effects, how high fat diet and hyperglycemia influence pancreatic expression profile of GLP-1 and its receptor remains to be clarified. An animal model of Type 2 Diabetes (T2DM) was generated using C57BL/6J mice
to study the alteration in GLP-1 expression profile induced by high fat diet and hyperglycemia. Obese mice fed with high fat diet (HFD) exhibited insulin resistance and glucose intolerance compared to lean mice fed with standard diet (SD). Hyperglycemia was induced by low dose streptozotocin (STZ) injection. Immunohistochemistry analysis of pancreatic tissues demonstrated low levels of GLP-1 and GLP-1R expressions in pancreatic islets of both obese and lean mice. Intriguingly, proglucagon cross-reactivity of anti GLP-1 antibodies resulted in strong staining of pancreatic alpha cells in both group. Future experiments are underway to reveal alterations of GLP-1 and GLP-1 receptor expression profile in HFD fed-STZ induced diabetic mice.
Financial support: This study is supported by Akdeniz University and the Scientific and Technological Research Council of Turkey (TUBITAK-112S114).