This study investigated the relationship between hyperglycemia and electrodermal activity ( EDA) parameters in streptozotocin ( STZ) induced diabetic rats. The article evaluates the course of development of neurophysiological alterations in the peripheral nervous system in diabetic rats through EDA. Rats were made diabetic using a moderate dose of STZ ( DI) and high dose ( DII). The placebo group ( P) was injected with physiological saline. EDA was recorded 1 h before the injection ( beginning, 0, day), 1st day ( one day after the injection) and 10th day. Skin conductance level ( SCL) was lower in DII than P on the 1st and the 10th days. The SCL and SC fluctuation rate ( SCFr) of DI were significantly lower on the 10th day compared to their first record. SC response rate ( SCRr) was lower on the 10th day compared to the 1st day, in the DI. In the DI, SCL, SC fluctuation rate ( SCFr) and SC response rate ( SCRr) were lower on the 10th day compared to the 1st day. The DII was statistically higher in electrodermal non-responsiveness compared to other groups on the 1st day. The results obtained show that hyperglycemia affects the peripheral nervous system, and EDA parameters are affected by blood glucose level. It is suggested that EDA is a simple and non-invasive electrophysiological method in early diagnosis of diabetic neuropathy.