EFFECTS OF GENOTYPIC VARIATION AND SOME NEW PROTOCOLS ON GYNOGENESIS EFFICIENCY OF CUCUMBER


PINAR H., Toprak S., HANCI F., Simsek D.

FRESENIUS ENVIRONMENTAL BULLETIN, vol.30, no.5, pp.4763-4770, 2021 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 30 Issue: 5
  • Publication Date: 2021
  • Journal Name: FRESENIUS ENVIRONMENTAL BULLETIN
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Environment Index, Geobase, Greenfile, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Page Numbers: pp.4763-4770
  • Erciyes University Affiliated: Yes

Abstract

In this study, gynogenesis capacity of different Cucumber germplasm, including some commercial varieties, was evaluated and optimized. Unfertilized female flowers of 23 hybrid cucumber genotypes were used as explant source after the two or three weeks of emergence. For each genotype, 30 Petri dishes were prepared with five explants per dish. The study was planned in three stages. In the first stage, the ovarian culture efficiency was tried to be determined by using three ovarian culture protocols which were used in previous studies and obtained successful results. The highest result of embryo unit for per Petri and callus unit for per Petri were obtained from protocol-1 (MS + 1 mg L-1 TDZ + 1 mg L-1 IBA) according to the average of all genotypes at this stage (1.57 units / Petri). In the second stage of the study, the best protocol selected from the first stage, and two new protocols (P-4 and P-5) created with modifications were tested. At the end of this stage, the best protocol selected from the first stage (P-1) and the P-4 (MS + NAA 1.5 mg L-1 + BAP 1.8 mg L-1 + 2,4D 0.7 mg L-1 + Kinetin 1.0 mg L-1) yielded the highest embryo results. In the last stage of the study, two new protocols were tried, but none of them were as good as the previously tried protocols. The results showed that the interaction between genotype x media components was found to be statistically significant at all stages.