The objective of this study was to identify allele and genotype frequencies of the kappa-CN and beta-LG genes in Anatolian water buffalo. A total of 126 water buffalos from Turkey were genotyped using the PCR-RFLP method. For gene kappa-CN, only B allele and BB genotype were observed. And for gene beta-LG, two types of alleles (A and B) and three types of genotypes were observed. The genotype frequencies of AA, AB and BB of beta-LG in Anatolian water buffalo were 0.254, 0.698 and 0.048, respectively. Surprisingly, the frequency of allele A was higher than that of allele B in contrast to world buffalo breeds.