Seventy accessions of the genus Citrus and related genera in Aurantioideae were used to better identify genetic diversity, estimate genetic similarities, polymorphism rates and relationships using amplification of minisatellite DNA (DAMD) markers. A total of 255 bands were scored from 20 DAMD-PCR markers and all (100%) of them were polymorphic. The accessions studied had similar values ranging from 0.31 to 0.84, showing a high level of variation. DAMD markers provided useful results to understand genetic basis of the citrus group. In addition, these markers revealed different knowledge from the other DNA-based marker system among the accessions. Also, DAMD-PCR markers appeared to be as useful as other for gerietic analysis in citrus and its relatives.