Flow-cytometric comparison of RNA content in peripheral blood mononuclear cells of down syndrome patients and control individuals


HAMURCU Z. , DEMIRTAS H., Kumandas S.

CYTOMETRY PART B-CLINICAL CYTOMETRY, no.1, pp.24-28, 2006 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Publication Date: 2006
  • Doi Number: 10.1002/cyto.20077
  • Title of Journal : CYTOMETRY PART B-CLINICAL CYTOMETRY
  • Page Numbers: pp.24-28
  • Keywords: Down's syndrome, trisomy 21, age-dependent RNA content, SYNDROME BRAIN, PROTEIN-LEVELS, CELLULAR DNA, EXPRESSION, GENES, CHROMOSOME-21, LYMPHOCYTES, TRISOMY-21, SURFACE

Abstract

Objective: Trisomy 21 or Down syndrome (DS) is the most common genetic cause of mental retardation associated with the immunologic and other known defects. Extra chromosome 21 of DS patients contains an average of 40 extra copies of rRNA genes and the in vivo regulation of these genes' activity is not known. Because over 80% of total cellular RNA is rRNA, the measurement of total cellular RNA provides information on rRNA content. The aim of this work was to determine whether or not the additional chromosome 21 causes any increase in total cellular RNA content in mononuclear cells from peripheral blood (PBMNCs) of these patients and whether or not this content is modified with age.