Development of a Colorimetric Urease Test Based on Au NPS Capped with Anthocyanin for the Rapid Detection of Helicobacter pylori through Multiple Readouts
ChemistrySelect, cilt.8, sa.27, 2023 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 8 Sayı: 27
- Basım Tarihi: 2023
- Doi Numarası: 10.1002/slct.202300637
- Dergi Adı: ChemistrySelect
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier
- Anahtar Kelimeler: Anthocyanin Capped Au NPs, Colorimetric Test, Urease Test, Helicobacter pylori, Multiple Readouts
- Erciyes Üniversitesi Adresli: Evet
Özet
In this work, we have reported use of anthocyanin capped gold nanoparticles (Ant@Au NPs)-incorporated colorimetric urease test for rapid, sensitive and economic detection of Helicobacter pylori (H. pylori). This test containing Ant@Au NPs, urea, sodium phosphate buffer (PBS) and sodium azide (NaN3) was prepared at pH 5 with red solution owing to dispersity of Ant@Au NPs, urease enzyme secreted from H. pylori cause production of ammonia (NH3) via hydrolysis of urea, then which makes reaction environment alkaline (pH 8.2). The limit of detection for this Ant@Au NPs based urease test was determined to be to 102 CFU/mL with proportional to increasing incubation time. However, distinct color change was observed with 104 CFU/mL H. pylori suspensions in 15 min. The anthocyanin molecules existing on surface of Ant@Au NPs were easily deprotonated from hydroxyl groups in the alkaline condition, which caused various changes on Ant@Au NPs including quite much negative charges on the surface of Ant@Au NPs analyzed by Zeta potential, aggregation of Ant@Au NPs demonstrated by STEM, DLS and spectrophotometer, and turning the color of test solution to purple evaluated by a naked eye and digital process imaging system.