Determination of Organochlorinated Pesticide Residues By Gas Chromatography - Mass Spectrometry after Elution in A Florisil Column


KOc F. , Karakus E.

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.17, ss.65-70, 2011 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 17 Konu: 1
  • Basım Tarihi: 2011
  • Dergi Adı: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Sayfa Sayıları: ss.65-70

Özet

The purpose of the present study was to develop an easy analytical method for determining alpha-hexachlorocyclohexane (alpha-HCH), hexachlorobenzene (HCB), heptachlor (HC), aldrin (ALD), 4,4-dichlorodiphenyldichloroethylene (4,4-DDE) and 4,4-dichlorodiphenyltrichloroethane (4,4-DDT) residues by gas chromatography with mass spectrometre (GC-MS) for routine analysis. Chicken muscles were utilized as samples in this study. A florisil packed column was used in sample preparation step and showed good performance. The average precision and accuracy ranged 8.03-24.00% and -4.36-30.67%, respectively (n=6). The average recoveries were between 95.74 and 130.67% for various spiking levels (25, 50, 100 ng/g). The average of inter- and intra-day precision was <13 and the limits of detection were ranged 7-19 ng/g, depending on different organochlorinated (OC) pesticides. In conclusion, the extraction method used in the present study was inexpensive (do not require skilled operators, high volume of solvent or costly apparatus), easy and rapid. Additionally, the validation parameters show that the proposed method in this study is sensitive, reproducible and reliable alternative to the normally used methods. Thus, it could efficiently be used in the routine and monitoring studies so that several samples can be run in parallel.