First time in this study, thymol was used as organic component for the synthesis of hybrid Cu- nanoflowers alternative to these expensive molecules in a pH dependent manner. Thymol initiated the coordination reaction with copper II ions, which act inorganically in the formation of Cu-NFs. Morphology of thymol based hybrid nanoflowers (T-hNFs) was observed with SEM images (pH 5). The presence of copper II ions, crystal structure and the presence of functional groups in the structure of T-hNFs have been shown by EDX, and FTIR graphs, respectively. Furthermore, T-hNFs were found to have antimicrobial properties (MIC = 25 μg/ml) against fish pathogens. In terms of the catalytic activity of T-hNFs, guaiacol was oxidized to product and its oxidation was reached to saturation point in 90 min by increasing absorbance point from 0.01 (first 5 min) to 0.031(at 90 min) with 3 fold increase.