Akademik Veri Yönetim Sistemi
14. INTERNATIONAL CONFERENCE ON AGRICULTURE, ANIMAL SCIENCE & RURAL DEVELOPMENT, İzmir, Türkiye, 22 - 24 Mart 2024, ss.27-28
In addition to increasing the production of livestock animals such as cattle, sheep and goats bred in our country, it is also important to select individuals with high productivity character and use them in breeding. Sheep are a highly special livestock animal due to their ability to utilize poor pasture and to withstand difficult climatic conditions. The main purpose of sheep breeding in Türkiye today is mutton production. In the production of mutton, the meat must not only be volumetric, but also meet international meat quality norms. Nowadays, determining the genes and their molecular mechanisms that affect quantitative characters that are economically important in animal breeding has become one of the most important goals of researchers. In this study, the relationship between the expression levels of fatty acid binding protein 4 (FABP4) and fatty acid synthetase (FASN) genes in some native sheep breeds raised in different regions of Turkey was investigated. The animal material for this study consisted of 5-month-old Kıvırcık (n=10), Karayaka (n=10), Akkaraman (n=10), and Awassi (n=10) breed lambs. RNA was isolated from longissimus dorsi tissue samples taken from the animals in the study group, and RT-qPCR analysis was performed to determine FABP4 and FASN gene expression levels. RNA was isolated from longissimus dorsi tissue samples taken from the animals in the study group, and RT-qPCR analysis was performed to determine FABP4 and FASN gene expression levels. In the statistical analysis performed at the study, FASN gene expression according to the fat tail phenotype was found to be statistically significant in the Akkaraman breed compared to the Karayaka breed(P<0.05). In the thin tail phenotype analysis, FABP4 and FASN gene expression were statistically significant in the Awassi breed compared to the curly breed (P<0.05). In the analyses conducted among sheep breeds, FABP4 and FASN gene expression was found to be statistically significant in all sheep breeds (P<0.05). In addition, while FABP4 gene expression is down-regulated in the Karayaka breed; It was upregulated in the Awassi breed. While FASN gene expression is upregulated in Awassi, Karayaka and Kıvırcık breeds; It was found to be downregulated in the Akkaraman breed. As a result, FABP4 and FASN gene expressions showed various significant differences between races. It is thought that these differences may be related to nutritional differences and genetic characteristics between races.
Keywords: FASN, FABP4, Gene expression, Sheep