Comparison of ELISA and flow cytometry for measurement of interleukin-1 beta, interleukin-6 and tumor necrosis factor-alpha


ÇETİN A., Sen A., Cetin I., ÇİMEN B., ÇİMEN L., Savas G., ...More

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, vol.43, no.5, pp.540-548, 2018 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 43 Issue: 5
  • Publication Date: 2018
  • Doi Number: 10.1515/tjb-2017-0164
  • Journal Name: TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.540-548
  • Keywords: Method comparison, Flow cytometry, ELISA, IL-1 beta, IL-6, TNF-alpha, HUMAN CYTOKINES, BEAD ARRAY, ASSAYS, SERUM, QUANTITATION, TECHNOLOGY, BIOMARKERS, GUIDELINES, REGRESSION
  • Erciyes University Affiliated: Yes

Abstract

Background: Although majority of the previous studies have shown a good correlation between enzyme linked immuno sorbent assay (ELISA) and flow cytometry in terms of cytokines, two laboratory methods usually were compared with the regression analysis and correlation in the literature. This study aimed at comparing the ELISA and flow cytometry assay for measuring cytokines by using two different statistical methods, regression analysis and Bland-Altman plot.