preparation of maltase-inorganic hybrid nanoflowers and investigation of inflence on enzymatic activity of synthesis conditions


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Somtürk Yılmaz B., Özdemir N.

1 st international conference on innovations in natural science and engineering, Gazimagusa, Kıbrıs (Kktc), 3 - 06 Ocak 2018

  • Yayın Türü: Bildiri / Yayınlanmadı
  • Basıldığı Şehir: Gazimagusa
  • Basıldığı Ülke: Kıbrıs (Kktc)
  • Erciyes Üniversitesi Adresli: Evet

Özet

Enzymes have found widespread in various scientific and technical fields, including chemistry, biochemistry, medicine, pharmaceutical science and industry [1]. Although enzymes have unique properties, such as high catalytic activity, reaction specificity, stability, selectivity, low toxicity and water solubility, their efficient use is very limited due to their innate disadvantages associated with instability during storage, loss of catalytic activity after  implementation into the reaction, high production cost and lack of recovery from reaction medium, substrate- and product-inhibitions [2].  In order to overcome these limitations, several immobilization approaches have been developed.

In this study,  maltase-copper phosphate hybrid nanoflowers (HNFs) synthesized and at the same time formation mecanism, catalytic activity and stability under different experimantal parameters (pHs, temperatures) was investigated. The hNFs were characterized using SEM, EDX analysis, Bradford assay, UV-Vis spectrometry and XRD. The effect of reaction temperatures on the morphology of the hNFs was demonstrated with SEM images (Fig. 1).