Genetic variability in peas (Pisum sativum L.) from Turkey asssessed with molecular and morphological markers

HANCI F.

FOLIA HORTICULTURAE, cilt.31, sa.1, ss.101-116, 2019 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31 Sayı: 1
  • Basım Tarihi: 2019
  • Doi Numarası: 10.2478/fhort-2019-0007
  • Dergi Adı: FOLIA HORTICULTURAE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.101-116
  • Anahtar Kelimeler: breeding, cluster analysis, diversity, pea, polymorphism, WILD-SPECIES ACCESSIONS, POPULATION-STRUCTURE, GERMPLASM COLLECTION, DIVERSITY, MICROSATELLITE, CULTIVARS, VARIETIES, GENOTYPES
  • Erciyes Üniversitesi Adresli: Evet

Özet

The aim of this study was to identify the molecular and morphological characteristics of Turkish pea accessions (Pisum sativum L.). The genetic diversity among 130 Turkish landraces and 2 commercial varieties in a total of 132 pea accessions was assessed with 14 simple sequence repeat (SSR) markers. Forty-eight (48) polymorphic alleles were identified using 14 SSR markers. The pairwise Dice coefficients of similarity between accessions ranged from 0.091 to 0.960. The polymorphism information content (PIC) value ranged from 0.585 to 0.861. Overall, 50 morphological traits were evaluated. Cluster analysis was carried out on a matrix of Euclidean distances. The accessions were divided into three main groups. Principal component analysis (PCA) was used to identify the weight of each morphological characteristic. According to the results, the highest eigenvalue was observed in PC-I (13.88) followed by PC-II (11.42), and PC-III (7.32). The first fifteen PCs with eigenvalues > 1 explained 74.08% of the variability. The results showed that the molecular markers were useful and polymorphic, sufficient to allocate all the evaluated accessions. This research has provided significant insights into the genetic variability of Turkish pea accessions.