Effects of dietary propolis on the number and size of pleopadal egg, oxidative stress and antioxidant status of freshwater crayfish (Astacus leptodactylus Eschscholtz)

Yonar S. M., Koprucu K., Yonar M. E., SİLİCİ S.

ANIMAL REPRODUCTION SCIENCE, cilt.184, ss.149-159, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 184
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1016/j.anireprosci.2017.07.010
  • Dergi Adı: ANIMAL REPRODUCTION SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.149-159
  • Anahtar Kelimeler: Antioxidant enzyme, Freshwater crayfish, Diet, Oxidative stress, Pleopadal egg, Propolis, TROUT ONCORHYNCHUS-MYKISS, SERIES FATTY-ACIDS, CHEMICAL-COMPOSITION, ANTIMICROBIAL ACTIVITY, PROTECTIVE ROLE, ANTIBACTERIAL, ANTIFUNGAL, TOXICITY, EXTRACTS, DEFENSE
  • Erciyes Üniversitesi Adresli: Evet

Özet

Four different crayfish diets; control, El, E2 and E3, respectively containing 0, 1, 2 and 4% propolis, were tested to determine the effects of dietary propolis on the number and size of pleopadal egg, and malondialdehyde (MDA) level, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities in the freshwater crayfish (Astacus leptodactylus). The crayfish were kept at 9.6 +/- 5.3 degrees C water temperature and fed three times daily during a six month period The pleopodal egg number (from 7 to 9) produced per gram of the bpdy weight and total pleopodal egg number (from 201 to 263) significantly increased (P < 0.05) with the dietary propolis supplementation. However, an increase in the dietary propolis led to a significant decrease (P < 0.05) in the pleopodal egg size (from 3.22 mm to 2.76 mm). MDA level significantly (P < 0.05) decreased in the hepatopancreas (from 4.78 to 3.04 nmol/g protein) and ovarium (from 3.52 to 1.98 nmol/g protein) of the crayfish fed with the increased dietary propolis level. On the other hand, an increase in the dietary propolis led to a significant increase (P < 0.05) in SOD activities in hepatopancreas (from 21.8 to 41.1 U/g protein) and ovarium (from 16.8 to 26.8 U/g protein). However, CAT activities significantly decreased (P < 0.05) in the hepatopancreas (from 23.8 to 18.9 nmol/g protein) and ovarium (from 21.8 to 17.5 nmol/g protein) of the crayfish fed with the increased dietary propolis level. Similarly, an increase in the dietary propolis caused a significant decrease (P < 0.05) in GSH-Px activities in the hepatopancreas (from 21.8 to 41.1 U/g protein) and ovarium (from 16.8 to 26.8 U/g protein) with the formation of the pleopodal egg. The dietary propolis improves reproductive efficiency in the crayfish and decreases the oxidative stress under controlled hatchery conditions.