The effect of Encephalitozoon intestinalis on oxidative stress and cytokine levels in U937 cells*


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Şentürk M. , Çetinkaya Ü. , Eren M. , Charyyeva A.

2nd International Conference on Veterinary Biochemistry and Clinical Biochemistry: Advances and Challenges in Understanding the Mechanism of Diseases, Ankara, Türkiye, 24 - 26 Ekim 2019, ss.28

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Ankara
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.28

Özet

Aim: Microsporidae, which can accommodated in many vertebrate and invertebrate organisms, are compulsory intracellular parasites that form spores. Encephalitozoon intestinalis (E.intestinalis) from the group of Microsporidae cause life-threatening diarrhea in immunosuppressed patients as well as; enteritis, small intestinal perforation, cholangitis, cholecystitis, nephritis, bronchitis, sinusitis, rhinitis, keratoconjunctivitis and disseminated infections. In this study, nitric oxide (NO), malondialdehyde (MDA), total antioxidant capacity (TAC), tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) levels were determined in U937 cells infected with E. intestinalis.

Material and Method: In the study, U937 human macrophage cell line and E. intestinalis 50506 (ATCC) strain were used. U937 cells were stimulated with Phorbol 12-Myristate 13-Acetate (PMA) and then infected with the parasite. Nitric oxide, MDA, TAC, TNF-α and IL-10 levels were determined in cell media collected at 6th, 12th and 24th hours after infection.

Results: Compared to the control group, NO levels at all time points and MDA levels at 24th hours were significantly increased, in the infected group. Total antioxidant capacity did not differ between the control and infected groups at the 6th and 12th hours, while the values of the infected group decreased at 24th hour statistically. There was no statistically significant difference in TNF-α values in both groups and at all time points. In the infected group compared to the control group, IL-10 levels showed a significant reduction only at 6th hours.

Conclusion: It was concluded that there was an increase in oxidant levels, a decrease in antioxidant levels, and no significant changes in cytokine levels in U937 cells infected with E. intestinalis.

 

Key words: Antioxidant, cytokines, Encephalitozoon intestinalis, oxidative stress.