Biological variation of peripheral blood T-lymphocytes

Falay M., Senes M., Korkmaz S., ZARARSIZ G., Turhan T., Okay M., ...Daha Fazla

JOURNAL OF IMMUNOLOGICAL METHODS, cilt.470, ss.1-5, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 470
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1016/j.jim.2019.04.002
  • Dergi Adı: JOURNAL OF IMMUNOLOGICAL METHODS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1-5
  • Anahtar Kelimeler: Biological variation, T lymphocytes, Flow cytometry, SUBSETS, ADULTS
  • Erciyes Üniversitesi Adresli: Evet

Özet

Background: Flow cytometric analysis of the lymphocyte subsets has become one of the most commonly used techniques in the routine clinical laboratory. It is frequently used in monitoring lymphocyte recovery after hematopoietic stem cell transplantation (HSCT), as well as diagnosis and treatment of acquired immunodeficiency syndrome (AIDS). Reliable biological variation (BV) data is needed for safe clinical application of these tests. In this study, similar preanalytical and analytical protocols to the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) checklist were followed and a stringent statistical approach was applied to define BV of T-lymphocytes.