Dynamic monitoring of the cytotoxic effects of <i>Cousinia iconica</i> extracts on A549 and RL95-2 cells using the xCELLigence RTCA system

Pasayeva L., KARABOĞA ARSLAN A. K., DEMİRPOLAT E., TUGAY O.

INDIAN JOURNAL OF TRADITIONAL KNOWLEDGE, sa.6, ss.573, 2024 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2024
  • Dergi Adı: INDIAN JOURNAL OF TRADITIONAL KNOWLEDGE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, Food Science & Technology Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Sayfa Sayıları: ss.573
  • Erciyes Üniversitesi Adresli: Evet

Özet

Asteraceae family plants have potential anticancer activity. In this study, the cytotoxic effects of C. iconica were investigated using different tumor cell lines: Non-small lung cancer (A549) and human endometrium carcinoma cell line (RL95-2). The cytotoxicity of the extracts was determined with MTT assay and the xCELLigence Real - Time Cell Analyser (RTCA). As a result, the ethyl acetate sub-extract and methanol extract of C. iconica were found cytotoxic to RL95-2 cell line with 271 and 51 mu g/mL IC 50 values, respectively. According to RTCA results, the IC 50 values of ethyl acetate subextract were 44.82, 60.89, and 55.22 mu g/mL and methanol extract 265.52, 250.48, and 246.65 mu g/mL, for 24, 48, and 72 h exposure, respectively. RL95-2 cells are more sensitive to ethyl acetate sub-extract than methanol extract. This study is the known first study to show the cytotoxic effects of C. iconica extracts on both A549 and RL95-2 cell lines.