Akademik Veri Yönetim Sistemi
FARMACIA, cilt.70, sa.2, ss.287-294, 2022 (SCI-Expanded, Scopus)
This study aimed to evaluate the antioxidant activity, the inhibitory effect on digestive enzymes linked to diabetes and the phytochemical composition of an active sub-extract obtained from Cousinia aintabensis (Asteraceae). The antioxidant effect of extracts was investigated with DPPH center dot, ABTS(center dot+) and FRAP tests and the inhibition effect was tested on alpha-amylase and alpha-glucosidase. The quantitative and qualitative determination of the compounds within the active extract was carried out by LC-MS/MS. The highest total phenolic (185.816 +/- 0.003 mg GAE/g extract) and total flavonoid content (147.423 +/- 0.003 mg CA/g extract) was found in ethyl acetate sub-extract of C. aintabensis (CAE). It also showed the highest antioxidant activity on DPPH center dot (IC50 = 0.473 +/- 0.056 mg/mL), ABTS(center dot+) (at a conc. of 0.5 mg/mL equivalent to 0.543 +/- 0.005 mu M Trolox) and FRAP (at a conc. of 1 mg/mL equivalent to 2586 +/- 6.421 mmol Fe2+) tests. Furthermore, the highest alpha-amylase and alpha-glucosidase activity were also found in CAE (IC50 = 0.361 +/- 0.010 mu g/mL for alpha-glucosidase and 1.010 +/- 0.010 for alpha-amylase). According to LC-MS/MS analysis, the main compounds of CAE were cynarin and isorhamnetin 3-O-rutinoside.