Helichrysum Mill., (Asteraceae) species have been used in folk medicine for thousands of years in the world. The In vitro cytotoxic effects in human lymphocytes of 6 Helichrysum taxa used in Turkey folk medicine were investigated. Blood samples were obtained from healthy donors, nonsmoking volunteers, which were incubated and exposed to increasing concentrations of methanol extracts of Helichrysum taxa (0.01, 0.05, 0.1, 0.5 and 1 mg/mL). According to results, other taxa outside H. peshmenianum and H. goulandriorum affected MN rates at high concentrations (0.5 and 1 mg/mL). However mitotic and replication indexes rates of H. pallasii and H. chionophilum did not show a cytotoxic effect.