Polyhedral oligomeric silsesquioxane (POSS) containing low-density polyethylene (LDPE) based innovative active nanocomposite food packaging films were produced using film blowing technique. Octa ammonium POSS (OA-POSS), N-Phenylaminopropyl POSS (AP-POSS), methacryl POSS (MA-POSS) were used as a functional additive. The effectiveness of active packaging films were mainly evaluated via Escherichia coli O157: H7 and native microflora (total mesophilic aerobic counts, lactic acid bacteria, total Enterobacteriaceae) growth on minced beef. The color properties, oxidative stability, and pH change in minced beef were also investigated. OA-POSS and AP-POSS containing films with vacuum packaging had an antibacterial effect on E. coli O157: H7 and native microflora breeding during the minced beef storage. Thiobarbituric acid (TBA) formation was inhibited by treatment of OA-POSS up to 7 days. Consequently, it might be recommended that active nanocomposite food packaging materials containing OA-POSS and AP-POSS could be used for the extension of the shelf life of the minced beef.