Glymphatic sistem


Nur İ. H.

İCAGAS 2018 (internatinal Congress on Agriculture and Animal Sicences, Antalya, Turkey, 7 - 09 November 2018, pp.168-185

  • Publication Type: Conference Paper / Full Text
  • City: Antalya
  • Country: Turkey
  • Page Numbers: pp.168-185
  • Erciyes University Affiliated: Yes

Abstract

There is no known system of lymphatic circulation in the brain. It is not clear how the solutes in the brain pass through the interstitial space in the brain parenchyma. A significant

portion of the CSF in the subarachnoid space has been proven to pass into the brain parenchyma. Glymphatic system is a recently discovered macroscopic waste cleaning

system that uses a unique perivascular channel system created by astroglial cells to effectively remove soluble proteins and metabolites from the central nervous system

Surprisingly, the glymphatic system functions mainly during sleep and is largely disabled during wakefulness. Therefore, the need for biological sleep among all species is an activity

that allows the brain to remove potentially neurotoxic waste products, including β-amyloid, from the middle. The Glymphatic system [or glymphatic interval or perivascular system] is

a functional waste removal pathway for the central nervous system [CNS]. The Glymphatic system is a mechanism for both intracellular and extracellular fluid cleansing. The

anatomical profile of the network is complex and is a structure supported by astrocytic aquaporin-4 (AQP4) water channels. Its role in the brain of this newly discovered system

is equivalent to the lymphatic system found in other body organs and is called "glymphatic pathway" or "[g] lymphatics" because of its dependence on glial cells. ERÜ. ICG was used as a fluorescent substance in the preliminary study of the project "Dental Investigation of Brain Interstitial Fluid (ISF) Using a Quantum Dot (Qdot) Based Biological Sensor Probe" prepared by the Department of Anatomy of the Veterinary Faculty. ERÜ. In accordance with the decision of the Local Ethics Committee (EÜHADYEK) dated 16.07.2018 and

numbered 18/095, 1-1.5 μl of ICG was injected into the left parietal lobe at a depth of 2- 2.5 mm with a Hamilton injector under general anesthesia and in-vivo images were taken.

In the left parietal lobe of the developed male rat brain, 1 μl injected 2 mm lateral and 2- 2.5 mm deep in the middle of the sinus sagittal dorsalis was injected for 20 minutes, 30

minutes, 40 minutes after ICG injection, and 60 min fluorescent in-vivo imaging images were taken in the right mandibular lymph nerve and then in the left mandibular lymph

nerve. The first signal following the injection showed itself on the right side. After 30 minutes, the signal became stronger. After about 40 minutes, the fluorescent image was started to be taken on the left side. From approximately 60 minutes, the image was obtained strongly on both sides. [SYNGENE G: BOX CHEMI XRQ (Hi-LED System) device used for

images

Keywords: Brain, Lymph drainage, Glymphatic system, Rat