Journal of Fluorescence, 2026 (SCI-Expanded, Scopus)
The exploitation of an extremely sensitive and reliable nanozyme based colorimetric sensor for highly sensitive detection of hydrogen peroxide (H2O2) in living body system is extensively carping owing to fact that it garnered a prime role in causing toxic diseases. In this work, a highly novel catalyst named as Cerium oxide (CeO2) nanosheets (NSs) was precisely prepared by using a simple one pot hydrothermal method, thereby dowered with a strong intrinsic peroxidase like capability of catalyzing the oxidation-reaction of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to generate a blue color reaction in the presence of H2O2. Hence, a sensitive visible assessment platform related to CeO2 NSs was engineered, which not only exhibited a wider detection range of 0.01–1000 µM with R2 value of 0.9998, but also perceives exceptional selectivity, tremendous cycling and long term stability. Further, the sensor also evaluates lowest detection limit (LOD) 0.29 ± 0.03 µM and quantification limit (LOQ) of 2.3 ± 0.03 µM. In addition, it also endorses impressive practicality with stronger sensitivity and favorable accuracy in serum samples. Thus, this research-work not only contributed upon CeO2 NSs an outstanding capacity to detect H2O2, but also expands the realm of applications for CeO2 NSs in the domains of environmental preservation, biomedical diagnosis and forensic sciences.