THE EFFECT OF DIFFERENT CONCENTRATIONS OF ACETOSYRINGONE ON TRANSFERRING rol GENES TO SOME CITRUS ROOTSTOCKS VIA Agrobacterium rhizogenes


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Dönmez D., Şimşek Ö. , Yalçın Mendi N. Y. , Yeşiloğlu T., Aka Kaçar Y.

Journal of Applied Biological Sciences, cilt.14, ss.281-295, 2020 (Diğer Kurumların Hakemli Dergileri)

  • Cilt numarası: 14 Konu: 3
  • Basım Tarihi: 2020
  • Dergi Adı: Journal of Applied Biological Sciences
  • Sayfa Sayıları: ss.281-295

Özet

Genetic  engineering  technologies  have  been  taken  part  in the breeding  strategies  of  fruit crops. Several biotechnological methods can be applied to plant to have better ones in the process of fruit breeding. Among these methods, Agrobacterium-mediated genetransfer is frequently used. Citrus species are the most widely grown fruit crops. Despite substantial genetic diversity and interspecific fertility, the genus  Citrus  includes  some  of  the  most  difficult  species  to  breed.  This  is  due  to  several  obstacles  for conventional  breeding.  For  this  reason,  biotechnological  breeding  methods  are  used  in  citrus  breeding. Acetosyringone  is  one  of  the  phenolic  compounds  secreted by  injured  plant  cells  and affectsthe transformation   efficiency.   This   study   aims   to   determine   the   effect   of   different   acetosyringone concentrations (0, 25, 50, 100 and 200 μM) on the transformation of rol genes in citrus genotypes and check whether the plantlets obtained after transformation carry the rolgenes by molecular methods. Tuzcu 3131 sour orange, Gou Tou sour orange, Carrizo citrange, Rough lemon, Volkameriana, Trifoliate orange were used  in  this  study.  As  a  result, the  highest  shoot  formation  was  obtained  at  200  μM  acetosyringone concentration and the highest root formation was obtained at 25 μM acetosyringone concentration