Akademik Veri Yönetim Sistemi
Meandros Medical And Dental Journal, cilt.26, sa.1, ss.19-32, 2025 (ESCI, TRDizin)
Objective: To investigate the expression levels of genes (SLC6A3, SLC6A4, SLC1A2, SLC18A2, MAOA, COMT, GLYAT, GRM5, DRD4, TPH1, and ADRA2C) associated with attention deficit hyperactivity disorder (ADHD) by pre and post-treatment with methylphenidate to see if they may serve as biomarkers in the etiopathogenesis of diseases. Materials and Methods: Thirty-five ADHD-diagnosed children and 38 healthy controls were included and divided three groups as control, pre-treatment and post-treatment group. Following total RNA isolation from participants' peripheral blood samples, cDNA was synthesized via reverse transcription and used for qPCR analyses. Results: Elevated SLC6A3 and decreased SLC6A4, SLC1A2, SLC18A2, ADRA2C, MAOA, COMT, GLYAT, DRD4 and TPH1 genes expression levels of childiren with ADHD were detected (p<0.01). In post-treatment group, while SLC6A3 and COMT expression levels decreased, the expression levels of SLC6A4, SLC1A2, SLC18A2, ADRA2C, MAOA, GLYAT, GRM5 and TPH1 significantly increased (p<0.01). Highlighted relationships among gene expressions levels of control, pre-treatment, and post-treatment groups were detected. Additionally, cut-off values with diagnostic power for SLC6A3, SLC6A4, SLC1A2, SLC18A2, ADRA2C, MAOA, COMT, GLYAT, DRD4, and TPH1 were detected. Conclusion: The expression levels of the SLC6A3, SLC6A4, SLC1A2, SLC18A2, ADRA2C, MAOA, COMT, GLYAT, DRD4, and TPH1 genes may play an important role in the formation, prognosis and etiopathogenesis of the disease, and these changes can also be used as biomarkers in the differential diagnosis and development of treatment strategies for the disease.