Role of CAPE on Protein Profile and Oxidative Stress Panel in Healthy Liver Tissue

KOÇOĞLU S. E., Çimen L., TUFANOĞLU N., YILMAZ S., Cetin A.

Journal of Basic and Clinical Health Sciences, cilt.9, sa.3, ss.457-464, 2025 (ESCI, TRDizin) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 9 Sayı: 3
  • Basım Tarihi: 2025
  • Doi Numarası: 10.30621/jbachs.1588609
  • Dergi Adı: Journal of Basic and Clinical Health Sciences
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.457-464
  • Anahtar Kelimeler: Propolis, caffeic acid phenethyl ester, liver, prooxidant, antioxidant, oxidative stress
  • Erciyes Üniversitesi Adresli: Evet

Özet

Purpose: We aimed to investigate the effects of caffeic acid phenethyl ester (CAPE), one of the active ingredients of propolis, on protein expression differences and some antioxidant parameters in rat liver. Material and Methods: 3 groups were formed as control (ip SF), CAPE 10 (ip 10 μmol/kg CAPE) and CAPE 50 (ip 10 μmol/kg CAPE for 5 days). Protein expression differences were analyzed by SDS-PAGE gel electrophoresis, GSH-Px, SOD enzyme activities and MDA levels were measured by ELISA. Results: The amount of protein with a molecular weight (MW) of 53 kDa in CAPE 10 was found to be higher than control group (p<0.05). The amount of protein with a MW of 35 kDa in CAPE 10 group was found to be higher than control (p<0.001). The amount of protein with a MW of 14 kDa in CAPE 50 group was found to be higher than control (p<0.05). MDA levels were statistically higher in CAPE groups compared to control group (p<0.001). GSH-Px and SOD activities in CAPE groups was found to be higher than control group, and the difference was not statistically significant. Conclusion: CAPE didn’t show the expected antioxidant effect on liver tissues, a prooxidant effect was observed.