South African Journal of Botany, cilt.194, ss.183-192, 2026 (SCI-Expanded, Scopus)
It is imperative to comprehend the genetic regulation of macro and micronutrient accumulation, given its pivotal role in enhancing the quality and productivity of crop production. The objective of this study was to identify molecular markers associated with the concentrations of macro (P, K, Ca, Mg, S) and micro (Fe, Zn, Cu, Mn, B, Na) nutrient elements in fruit tissues of 92 Recombinant Inbred Lines (RILs) derived from a cross between Capsicum annuum and Capsicum frutescens. A wide variation in macro and micronutrient contents was detected among the lines. Out of a total of 268 primer combinations belonging to the SRAP, ISSR, RAPD, and SSR marker systems, 30 primer combinations that exhibited polymorphism were selected, and a total of 164 markers were scored using these primers. The General Linear Model (GLM) was utilised for the purpose of conducting association analysis, which resulted in the identification of a total of 25 markers that were found to be significantly associated. A variable number of markers associated with plant nutrient elements were identified, and the proportion of phenotypic variance explained (R²) differed between elements. Seven markers for sodium (Na), five for calcium (Ca), one for potassium (K), one for phosphorus (P), two for iron (Fe), one for boron (B), and eight for zinc (Zn) were identified. This study represents one of the first examples in literature to map genetic regions controlling nutrient accumulation in pepper fruits and offers an important tool for biofortification and breeding programs utilizing molecular markers.