Effect of the influenza A (H1N1) live attenuated intranasal vaccine on nitric oxide (FENO) and other volatiles in exhaled breath


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Mashir A., Paschke K., van Duin D., Shrestha N., Laskowski D., Storer M., ...Daha Fazla

JOURNAL OF BREATH RESEARCH, cilt.5, 2011 (SCI İndekslerine Giren Dergi) identifier identifier identifier

Özet

For the 2009 influenza A (H1N1) pandemic, vaccination and infection control were the main modes of prevention. A live attenuated H1N1 vaccine mimics natural infection and works by evoking a host immune response, but currently there are no easy methods to measure such a response. To determine if an immune response could be measured in exhaled breath, exhaled nitric oxide (FENO) and other exhaled breath volatiles using selected ion flow tube mass spectrometry (SIFT-MS) were measured before and daily for seven days after administering the H1N1 2009 monovalent live intranasal vaccine (FluMist (R), MedImmune LLC) in nine healthy healthcare workers (age 35 +/- 7 years; five females). On day 3 after H1N1 FluMist (R) administration there were increases in FENO (MEAN +/- SEM: day 0 15 +/- 3 ppb, day 3 19 +/- 3 ppb; p < 0.001) and breath isoprene (MEAN +/- SEM: day 0 59 +/- 15 ppb, day 3 99 +/- 17 ppb; p = 0.02). MS analysis identified the greatest number of changes in exhaled breath on day 3 with 137 product ion masses that changed from baseline. The exhaled breath changes on day 3 after H1N1 vaccination may reflect the underlying host immune response. However, further work to elucidate the sources of the exhaled breath changes is necessary.