Copy For Citation
YUVALI ÇELİK G., ONBAŞLI D., ALTINSOY B., ALLI H.
European Journal of Medicinal Plants, vol.4, no.6, pp.709-722, 2014 (Peer-Reviewed Journal)
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Publication Type:
Article / Article
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Volume:
4
Issue:
6
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Publication Date:
2014
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Journal Name:
European Journal of Medicinal Plants
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Page Numbers:
pp.709-722
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Erciyes University Affiliated:
Yes
Abstract
Aim: To determine antimicrobial and antioxidative effects of Ganoderma lucidum.
Place and Duration of Study: Erciyes University, Faculty of Pharmacy, Pharmaceutical
Biotechnology research laboratory,Kayseri, Turkey, between January to March, 2013.
Methodology: Antimicrobial inhibitory effects were carried out on the extracts using disc
diffusion method. Antioxidant activities of the ethanolic and methanolic extracts from
G.lucidum were evaluated by using 2,2-diphenyl-1-picrylhydrazyl [DPPH] radical
scavenging, metal chelating, total flavonoid and total antioxidant activity assays. In
addition, the amounts of phenolic compound, β-carotene and lycopene components in the
extracts were determined.
Results: The antimicrobial effects of ethanolic and methanolic extracts of G. lucidum
were tested against one species of Gram positive bacteria, two species of Gram-negative
bacteria and two yeast. The highest inhibitory activity was determined against Candida
glabrata RSKK 04019 [25±1 mm, inhibition zone diameter]. On the other hand, the lowest
inhibitory activity was determined against Candida albicans ATCC 90028 and Bacillussubtilis RSKK 244 [10±1 and 10±0 mm, inhibition zone diameter]. DPPH radical
scavenging effect was detected in the methanol extract [IC50 = 3.82±0.04 μg/mL] was
higher than the ethanol extracts [IC50 = 7.03±0.07 μg/mL]. Compared to reference
antioxidant, the methanol and ethanol extracts of G.lucidum provided a lower IC50 than
butylated hydoxyanisole [BHA] [IC50 = 0.30±0.01 μg/mL]. Phenolic compounds were the
major antioxidant component found in the mushroom extracts.
Conclusion: These results showed that G. lucidum may be used in pharmaceutical
applications because of its effective antioxidant properties.