In this study, groundwater of 10 union councils of Larkana city was assessed by using atomic absorption spectrometry following microwave-assisted digestion. A total of 110 groundwater samples were collected by using a Global Positioning System. The results revealed that iron, zinc, nickel, copper, and lead present in the groundwater of Larkana city were within the level of 0.002-0.6 mg/L, 0.02-0.42 mg/L, 1-14.0 mg/L, 0.01-0.87 mg/L, and 0.01-12.0 mg/L, respectively. A total of 38% and 16% of samples showed a higher concentration of iron and lead, respectively. While other elements in the analyzed samples were within the safe limits set by WHO guidelines.