Induction of the mesenchymal stem cell to the skeletal muscle cell by the effect of growth factors

Doğan B., Kaan D., Ülger H.

Bratislava Medical Journal-Bratislavske Lekarske Listy, vol.121, 2020 (Journal Indexed in SCI Expanded)

  • Publication Type: Article / Article
  • Volume: 121
  • Publication Date: 2020
  • Title of Journal : Bratislava Medical Journal-Bratislavske Lekarske Listy




OBJECTIVES: The objektive of this study was to evaluated the differantitaion of human adipose tissue derived mesenchymal stem cells (MSCs) into skeletal muscle cells by the effect of IGF1 and FGF2 growth factors.

METHODS: MSCs were divided into four groups. (control group) was incubated only in myogenic medium,  II. group was incubated by adding 100 ng/ml FGF2 to the myogenic medium, III. group was incubated by adding 20 ng/ml IGF1 to the myogenic medium, IV. group was incubated by adding 100 ng/ml FGF2 and 20 ng/ml IGF1 to the myogenic medium. Cells dyed with MyoD1 antibody were analyzed by flow cytometry so as to determine myogenic differentiation at the end of 28 days.

RESULTS: It was confirmed that 11.5% of the control group, 39.2% of the FGF2 group, 23.1% of the IGF1 group and 39.3% of the IGF1-FGF2 group showed differentiation.

CONCLUSION: Our results show that IGF1 and FGF2 have a positive contribution on myogenic differentiation of MSCs, contribute to the studies related to muscle diseases and treatment of these diseases to support by growth factors the feature of the regeneration capacity and differentiation.

KEYWORDS: Stem Cell, Myogenic Differentiation, IGF, FGF